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KMID : 0895820140240030099
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Journal of Oriental Rehabilitation Medicine
2014 Volume.24 No. 3 p.99 ~ p.110
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Anti-oxidation and Anti-inflammatory Effect of Asiasari Radix in RAW 264.7 Cells
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Lee Ok-Jin
Oh Min-Seok
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Abstract
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Objectives : The purpose of this study was to investigate the Anti-oxidation and anti-inflammatory effects of ethanol extract from asiasari radix (AR) on lipopolysaccharide (LPS)-induced in RAW 264.7 Cells
Methods : Anti-oxidative effects of AR were measured by scavenging activities of 1,1-diphenyl- 2-picryl-hydrazyl (DPPH), 2,2``-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and production of reactive oxygen species (ROS) in RAW 264.7 cells. Anti-inflammatory effects of AR were measured by mediators including nitric oxide(NO), interleukin- 1β (IL-1β), interleukin-6 (IL-6), tumor necosis factors-α (TNF-α) and iNOS, IL-1β, IL-6, TNF-α mRNA expression in RAW 264.7 cells.
Results : Total phenolic content was expressed 28.77±1.67. DPPH radical Scavenging was increased depend on AR ethanol extract. ABAT radical Scavenging was increased depend on AR ethanol extract. Production of ROS was significantly decreased by AR ethanol extract on concentration of 100 (μg/ml). Production of NO was significantly decreased by AR ethanol extract on concentration of 100 (μg/ml). Production of IL-1β, interleukin-6 and TNF-α were increased depend on AR ethanol extract. And Production of interleukin- 6, TNF-α were significantly decreased AR ethanol extract. iNOS, IL-1β, IL-6, TNF- α mRNA expression of RAW 264.7 cells was increased depend on AR ethanol extract.
Conclusions : According to this study, AR ethanol extract has anti-oxidative and anti-inflammatoy effects.
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KEYWORD
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Asiasari radix (AR), Anti-oxidation, Anti-inflammatory, RAW 264.7 cell
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